Comments (4)
It is hard to say but we think there should be improvement. Based on our results (see Supplementary Table 3 in https://arxiv.org/pdf/2306.03399v1.pdf), 20x-30x UL reads with minimum length of 50kb could produce decent assemblies.
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Thanks for the quick response - much appreciated! Do you have an recommendations about minimum QV (quality values) for the reads?
Again, thanks for the help,
Jeff
from hifiasm.
I have no idea about that... But I feel like the new UL data should be fine?
from hifiasm.
Thanks - much appreciated.
Jeff
from hifiasm.
Related Issues (20)
- Help!!! Segmentation fault (core dumped) HOT 1
- Question about the depth of ONT ultra-long reads HOT 1
- Homotetraploid, super-large genome, with different parameters, the size of p_utg varies greatly? HOT 2
- setting K parameter in yak HOT 2
- how to make the correct genome size estimation for allotetraploid species? HOT 2
- Possible missing one haplotype in human assemblies HOT 2
- No haploid.gfa files output in trio-binning mode HOT 3
- Hifi + Hi-c + ONT assembly fails
- In Trio-binning, always more on hap1 despite (almost) same sequences for paternal and maternal
- discontinuous assembly with shorter pacbio hifi reads but high coverage HOT 2
- Is x20 of Hifi data enough to construct draft assembly of 6.5Gb genome? HOT 1
- line 8: 110334 Aborted(core dumped) HOT 1
- Ultra Long intergration failed: no output for UL kmer counting HOT 3
- missing 8Mb sequences in the assembly HOT 6
- Empty haplotype 2 gfa files by ONT integration HOT 1
- Basic Question About HiFi Input HOT 3
- Spend too long times to run hifiasm HOT 6
- Switch error on X and Y chromosome HOT 2
- *.ovlp.bin file HOT 1
- Resolving switching error (?)
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