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Comments (5)

Fu-Yilei avatar Fu-Yilei commented on August 23, 2024

Please attach you command for meth_phaser_parallel, thanks

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liuy0421 avatar liuy0421 commented on August 23, 2024

Something along the lines of the following:

meth_phaser_parallel \
-b haplotagged.bam \
-r Mus_musculus.GRCm39.dna.primary_assembly.fa \
-g phased.gtf \
-vc output.phased.vcf \
-o output/ \
-t $SLURM_CPUS_PER_TASK

I did not get errors for the autosomes, sex chromosomes, and 3 other unlocalized genomic contigs. This is a minor issue for my purpose for now because if I had filtered the input .vcf for only autosomes this wouldn't have been an issue.

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Fu-Yilei avatar Fu-Yilei commented on August 23, 2024

Sorry, I have 0 knowledge about mouse datasets, but I would recommend only using MethPhaser on autosomes since random x inactivation. If you could give me more information on your dataset I might be able to look into it further. Additional helpful information could be reads, vcf, etc.. However, I recommend only using it on autosomes.

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liuy0421 avatar liuy0421 commented on August 23, 2024

Makes sense - thanks a lot!

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Mar10L avatar Mar10L commented on August 23, 2024

Hi.
I am getting the same error:

Traceback (most recent call last):
  File "/iga/scripts/dev_modules/mambaforge/envs/duet-v0.6/bin/methphasing", line 1471, in <module>
    main(sys.argv[1:])
  File "/iga/scripts/dev_modules/mambaforge/envs/duet-v0.6/bin/methphasing", line 1396, in main
    [0], phased_region_list[1][0])]
IndexError: list index out of range

The command that I used was this one:

meth_phaser_parallel -t 10 -ml -2 \
-o methphaser \
-b grapevine.minimap2.filtered_only_primary.bam \
-r reference.fasta \
-g grapevine_phased.whatshap.stats.gtf \
-vc grapevine.clair3.phased.vcf.gz

Theoretically, I should not have problems with sex chromosomes, given the fact that grapevines do not possess them.

Thank you in advance for the help.

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