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slowkow avatar slowkow commented on May 23, 2024

I was able to reproduce your error.

If we look at the assignment column, we can see the issue is the presence of 5 unused categories:

[ins] In [8]: adata.obs['assignment'].value_counts().shape
Out[8]: (35,)

[ins] In [9]: (adata.obs['assignment'].value_counts() > 0).sum()
Out[9]: 30

We can use remove_unused_categories() to resolve the issue:

[ins] In [10]: adata.obs['assignment'].cat.remove_unused_categories(inplace = True)

[ins] In [11]: hm_obj = hm.run_harmony(adata.obsm['X_pca'], adata.obs, ['assignment'])
2021-05-17 10:41:38,184 - harmonypy - INFO - Iteration 1 of 10
2021-05-17 10:41:39,243 - harmonypy - INFO - Iteration 2 of 10
2021-05-17 10:41:40,278 - harmonypy - INFO - Converged after 2 iterations

from harmonypy.

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