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View Code? Open in Web Editor NEWGenome track visualization in your web browser, from R - a Bioconductor package
License: MIT License
Genome track visualization in your web browser, from R - a Bioconductor package
License: MIT License
Hi!
I am trying to automate plotting of many seg files in IGV using R and trying out your package. Thank you for developing this tool!
I tried to follow the Introduction: a simple demo vignette found here, and encountered an error when running displayTrack(igv, track)
. Please see the screenshot. I am not sure what it refers to or where to start troubleshooting.
The exact code I was executing is:
library(igvR)
igv <- igvR()
setBrowserWindowTitle(igv, "simple igvR demo")
setGenome(igv, "hg38")
showGenomicRegion(igv, "MYC")
loc <- getGenomicRegion(igv)
tbl.bed <- data.frame(chrom=loc$chrom, start=loc$start + 2000, end=loc$end-2000,
name="simple.example", stringsAsFactors=FALSE)
track <- DataFrameAnnotationTrack("simple bed", tbl.bed, color="random")
displayTrack(igv, track)
I am connected to RStudio on remote server through ssh with X11 forwarding enabled. I am also attaching the session info - am I missing any dependencies to run the igvR package?
> sessionInfo()
R version 3.6.3 (2020-02-29)
Platform: x86_64-centos7-linux-gnu (64-bit)
Running under: CentOS Linux 7 (Core)
Matrix products: default
BLAS: /gsc/software/linux-x86_64-centos7/R-3.6.3/lib64/R/lib/libRblas.so
LAPACK: /gsc/software/linux-x86_64-centos7/R-3.6.3/lib64/R/lib/libRlapack.so
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8 LC_PAPER=en_US.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] parallel stats4 stats graphics grDevices utils datasets methods base
other attached packages:
[1] igvR_1.6.1 BrowserViz_2.8.0 httpuv_1.5.4 jsonlite_1.7.1
[5] GenomicAlignments_1.22.1 Rsamtools_2.2.3 Biostrings_2.54.0 XVector_0.26.0
[9] SummarizedExperiment_1.16.1 DelayedArray_0.12.3 BiocParallel_1.20.1 matrixStats_0.59.0
[13] Biobase_2.46.0 GenomicRanges_1.38.0 GenomeInfoDb_1.22.1 IRanges_2.20.2
[17] S4Vectors_0.24.4 BiocGenerics_0.32.0 ggthemes_4.2.4 forcats_0.5.1
[21] stringr_1.4.0 dplyr_1.0.5 purrr_0.3.4 readr_1.4.0
[25] tidyr_1.1.2 tibble_3.0.4 ggplot2_3.3.2 tidyverse_1.3.0
loaded via a namespace (and not attached):
[1] bitops_1.0-6 fs_1.5.0 lubridate_1.7.9.2 bit64_4.0.5
[5] progress_1.2.2 httr_1.4.2 tools_3.6.3 backports_1.1.9
[9] R6_2.5.0 seqLogo_1.52.0 DBI_1.1.0 splitstackshape_1.4.8
[13] colorspace_2.0-1 withr_2.3.0 prettyunits_1.1.1 tidyselect_1.1.0
[17] curl_4.3 bit_4.0.4 compiler_3.6.3 cli_2.5.0
[21] rvest_0.3.6 xml2_1.3.2 rtracklayer_1.46.0 scales_1.1.1
[25] askpass_1.1 rappdirs_0.3.1 digest_0.6.27 pkgconfig_2.0.3
[29] BSgenome_1.54.0 dbplyr_2.0.0 rlang_0.4.10 readxl_1.3.1
[33] rstudioapi_0.13 RSQLite_2.2.1 generics_0.1.0 VariantAnnotation_1.32.0
[37] RCurl_1.98-1.2 magrittr_2.0.1 GenomeInfoDbData_1.2.2 Matrix_1.2-18
[41] Rcpp_1.0.5 munsell_0.5.0 lifecycle_1.0.0 stringi_1.5.3
[45] zlibbioc_1.32.0 Rtsne_0.15 BiocFileCache_1.10.2 grid_3.6.3
[49] blob_1.2.1 randomcoloR_1.1.0.1 promises_1.1.1 crayon_1.4.1
[53] lattice_0.20-41 haven_2.3.1 GenomicFeatures_1.38.2 hms_0.5.3
[57] pillar_1.4.7 biomaRt_2.42.1 reprex_0.3.0 XML_3.99-0.3
[61] glue_1.4.2 V8_3.4.2 data.table_1.13.2 BiocManager_1.30.10
[65] modelr_0.1.8 vctrs_0.3.7 cellranger_1.1.0 openssl_1.4.3
[69] gtable_0.3.0 assertthat_0.2.1 broom_0.7.2 later_1.1.0.1
[73] MotifDb_1.28.0 AnnotationDbi_1.48.0 memoise_1.1.0 cluster_2.1.0
[77] ellipsis_0.3.1
Hi,
My reference files and index files both sit on the server I am working on. I was wondering if there is a way for me to use them without giving url but instead giving path to the file which contains the reference fasta. How do I go about it for igvR?
Thank you
Saad
Nice presentation, Paul! In the rendered html, e.g., https://bioconductor.org/packages/devel/bioc/vignettes/igvR/inst/doc/basicIntro.html, the sessionInfo()
does not display because the closing 'fence'
Line 125 in ceb702e
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