Comments (10)
Hi, @HalfPhoton
I try pod5 convert fast5
command on the same sample as before, and it runs successfully. Thank you very much !
from pod5-file-format.
@HalfPhoton , thank you so much. I will try it. :)
from pod5-file-format.
it may be related to #33
Or maybe can you check how large your dataset is and whether there is enough diskspace available (in the working directory)?
from pod5-file-format.
Hi, @sklages . Thank you for your reply.
It seems not the memory or disk problem.
This is the detail about the error. When converting 30%, the speed is fast. But when converting 80%, the speed is quite slow, and when I top
, there is no pod5 command running.
from pod5-file-format.
@Flower9618 - the same here. And you're right, it's not a disk space issue. After all I came to the same conclusion. #33
from pod5-file-format.
Hi, @sklages .
Thank you very much. I see. So it seems even if I set the --threads 1
, it will not work. The only way to do it is breaking fast5 files into chunks...but this is inconvenient for large amounts of data...:(
from pod5-file-format.
Thanks for bringing this to our attention.
There are a number of changes to convert fast5 which are review which should hopefully improve performance. Including removing the initial multi-read fast5 check stage.
Thank you for your patience with us while we resolve these issues.
We'll let you know as soon as we get a tagged release out.
Kind regards,
Rich
from pod5-file-format.
@HalfPhoton .
Thank you so much. I am looking forward to it.
from pod5-file-format.
@Flower9618, I'm pleased to say we released 0.1.19
last night, which should contain fixes for your issue. Could you please give it a go and let us know if this patch has resolved your issues?
Kind regards,
Rich
from pod5-file-format.
@Flower9618 , I'm very happy to hear that! Thanks for letting us know that it's fixed ๐
Thanks again,
Rich
from pod5-file-format.
Related Issues (20)
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- Scratch/tmp pod5 problem HOT 21
- Semaphore hissy fit at the end of subset run HOT 1
- pod5 subset/filter in preparation for dorado duplex is slow HOT 5
- error with pod5 convert to_fast5 HOT 1
- Cannot install pod5 through pip on ARM due to dependency issues HOT 11
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- getrandom error with pod5 convert fast5 HOT 14
- MantaControl': Unable to read fast5 file at /path/: HDF5 exception", HOT 2
- Getting the signal chunk size of a pod5 file HOT 1
- Missing conda pod5 package HOT 1
- No documentation regarding multi-file pod5 dependency HOT 2
- pod5 convert fast5 warning: Failed to read key read_XXX HOT 2
- Troubleshooting Conversion of Fast5 Files to Pod5 Format HOT 12
- error๏ผXX.fast5 is not a multi-read fast5 file HOT 2
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