dRep is a python program for rapidly comparing large numbers of genomes. dRep can also "de-replicate" a genome set by identifying groups of highly similar genomes and choosing the best representative genome for each genome set.

Manual, installation instructions, and API are at available at ReadTheDocs

Publication is available at ISMEJ

Open source pre-print publication is available at bioRxiv

$ pip install drep

$ dRep compare output_directory -g path/to/genomes/*.fasta

$ dRep dereplicate output_directory -g path/to/genomes/*.fasta

$ dRep check_dependencies

• Mash - Makes primary clusters (v1.1.1 confirmed works)
• MUMmer - Performs default ANIm comparison method (v3.23 confirmed works)

• fastANI - A fast secondary clustering algorithm
• CheckM_ - Determines contamination and completeness of genomes (v1.0.7 confirmed works)
• gANI (aka ANIcalculator) - Performs gANI comparison method (v1.0 confirmed works)
• Prodigal - Used be both checkM and gANI (v2.6.3 confirmed works)
• NSimScan - Only needed for goANI algorithm (open source version of gANI)