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bd_projects_bulkseq's Introduction

BD_projects

This is a project about Behcet's disease I also would like to package some normal useful packages into this projects.

#File introduction: **/script ** #Completed computational propress under this folder.

  1. User can use 1.DEseqDataset_and_batcheffect.Rmd to explore batch effect in samples.

  2. When make the batch information clear, User can generate DESeqDataSet by 2.Generate_DESeqDataSet.R

2.5 Then User can generate normal result or figure by 2.5 Main.R.

  1. User can also calculate correlation between gene and clinical index by 3. Clinical_coranalysis.R

  2. User can generate immune cells proportion from https://cibersort.stanford.edu/ and then analysis more details by 4.CIBERSOFT.analysis.R.

  3. Then, If required, users can draw a Venn diagram of different genes in different situations。

/data User can find all test data under this folder. Reference:

  1. https://ycl6.gitbooks.io/rna-seq-data-analysis/quantification_using_rsem1.html
  2. http://tiramisutes.github.io/2018/12/04/ref-RNA-seq.html
  3. https://combine-australia.github.io/RNAseq-R/06-rnaseq-day1.html (downstream analysis)

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