Comments (2)
If you only want to identify the novel translating ORFs, PCR duplication removal is not necessary.
PCR duplications might influence the quantification of the translational level of ORFs。
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Get it! Thanks for your reply!
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Related Issues (20)
- Change readme from pip to pip3 HOT 1
- Error in GTFupdate HOT 1
- psite HDF5 file is saved in the wrong location HOT 2
- psites HDF5 file fails to be created HOT 1
- prepare_transcripts not taking multiple CDSs into account HOT 1
- ValueError: Can't transform the genomic interval, please check! HOT 1
- Read counts issues HOT 2
- ImportError: cannot import name 'PPoly' from partially initialized module 'scipy.interpolate' (most likely due to a circular import) (/home/zhai2/miniconda3/lib/python3.8/site-packages/scipy/interpolate/__init__.py) HOT 1
- plot_orf_density error,it show the following arguments are required: --start-codon
- No obviously periodicity are detected from bam file, it could be due to poor quality sequencing. Please check the metagene plots and try again by lowering the value of frame0_percent HOT 1
- can't locate attribute: psites_number HOT 1
- RiboCode_onestep crashes when calling detectORF HOT 1
- Whether multiple mapping affect ribocode result HOT 1
- error in metaplots step HOT 1
- 请问关于ORF预测结果中‘Psites_frame0_RPKM’代表什么意思? HOT 4
- Empty "transcripts_cds.txt" file HOT 1
- TypeError: '<' not supported between instances of 'str' and 'NoneType' HOT 2
- Question on adjusted P values reported in collapsed output HOT 2
- different transcripts of one gene
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