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Panqiao Wang's Projects

primer-algorithm icon primer-algorithm

A primer design algorithm for Multiplex Polymerase Chain Reaction (PCR).

primer-designer icon primer-designer

Design specific qPCR primers using Multiple Sequence Alignments as input.

primer-way icon primer-way

Overlapped primer design for entire coding sequence of the gene with specificity check

primer3 icon primer3

Primer3 is a command line tool to select primers for polymerase chain reaction (PCR).

primer3-1 icon primer3-1

This is a clone of an SVN repository at http://svn.code.sf.net/p/primer3/code. It had been cloned by http://svn2github.com/ , but the service was since closed. Please read a closing note on my blog post: http://piotr.gabryjeluk.pl/blog:closing-svn2github . If you want to continue synchronizing this repo, look at https://github.com/gabrys/svn2github

primer3_tab icon primer3_tab

cgi scripts for a very simple primer3 interface and parsing output to tab-delimited

primer_tool icon primer_tool

This is a simple program that reads in a file with multiple sequences in fasta format and then finds the primers that are required to join these sequences together using a yeast assembly protocol. Dependency - primer3 (https://github.com/libnano/primer3-py)

primerblast icon primerblast

This is a python script that allows the user to enter two genomic primer sequences and retrieves the sequence in between them.

primerblast_multiplexer icon primerblast_multiplexer

Script to identify groups of primers that are not predicted to heterodimerise (dG >-7000cal, at 37 degrees, as calculated by primer3) using the primer outputs of primerBLAST

primerdesign icon primerdesign

A tool to design highly specific PCR primers for the validation of genomic alterations including structural variants

primerdesign-3 icon primerdesign-3

Design primer for a fasta sequence, or a list of gene when genome sequence and gff3 file is provided

primerdesigner icon primerdesigner

A quick tool for automating primer3 design and primer specificity check workflow

primermapper icon primermapper

GUI for batch primer design with graphical output for PCR and SNP detection

primerseq icon primerseq

Primer Seek in RNA-Seq. Designs RT-PCR primers that validate alternative splicing events from RNA-Seq data.

primerview icon primerview

high-throughput primer design and graphical visualization

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