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seq_iics's Introduction

GET_CONSENSUS (AMPLICONES)

[TOC]

Run pipeline via nextflow. Notes on building and pushing Docker image are here

REQUIREMENTS

  • nextflow
  • docker

SETUP

data in data/input containing

  • barcoded | demultiplexed fastq files
  • your_samplesheet.tsv
  • your_reference.fasta
  • a config.json file with the following structure
{
	"sample": "your_samplesheet.tsv",
    "reference": "your_reference.fasta"
}

Execute on linux or wsl terminal

git clone 'https://github.com/Seq-IICS/seq_iics.git'
cd seq_iics
mkdir -p data/input
# copy your data to project data/input directory
nextflow run main.nf --data {myDataDir} -with-docker vici0uz/iics:latest

Tools used

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