[TOC]
Run pipeline via nextflow. Notes on building and pushing Docker image are here
- nextflow
- docker
data in data/input containing
- barcoded | demultiplexed fastq files
- your_samplesheet.tsv
- your_reference.fasta
- a config.json file with the following structure
{
"sample": "your_samplesheet.tsv",
"reference": "your_reference.fasta"
}
Execute on linux or wsl terminal
git clone 'https://github.com/Seq-IICS/seq_iics.git'
cd seq_iics
mkdir -p data/input
# copy your data to project data/input directory
nextflow run main.nf --data {myDataDir} -with-docker vici0uz/iics:latest