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TobiTekath avatar TobiTekath commented on September 13, 2024

Hi @gadepallivs,

thank you for reaching out and please excuse the very late reply.

I have to admit, that I can not completely follow your thoghts above. Could you provide me with some more information?

I assume you have single-cell RNAseq data and have already clustered the single cells and performed a dimensional reduction?
And now you want to create a plot of transcript expression across those clusters?

My initial ideas would be to either overlay the transcript expression fractions over a dim. reduction plot to see if any cluster stands out. Alternatively the barplot should be easy to create, just with the transcript quantification data (you would not necessarily need DTUrtle for that). Could you tell me which steps of the analysis you already performed?

Best,
Tobi

from dturtle.

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