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stemangiola avatar stemangiola commented on September 24, 2024

This was solved with the new versions of tidybulk and SummarizedExperiment soon to be merged.

I hope your PhD is going well.

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jianwu1 avatar jianwu1 commented on September 24, 2024

@stemangiola
Hi Stefano,

Thanks! It's going ok. Hope you had a wonderful trip to Italy!

We just got our scRNAseq data. I'm trying to convert the bcl files into fastq files but found that the SampleSheet.csv file:
(for example: https://github.com/YichaoOU/HemTools/blob/master/docs/gallery/SampleSheet.csv )
that contains information such as the adapter, flow cell lane, etc are missing. Can I ask if I should ask WEHI sequencing people for this file or if we could create this file myself in order to use bcl2fastq?

Here is the data folder given by WEHI sequencing staff:
Screen Shot 2022-06-27 at 10 15 04 am

Kind regards,

Jian

from tidybulk.

stemangiola avatar stemangiola commented on September 24, 2024

Hello Jian,

better we bring this conversation to email, as it is not really about tidybulk.

My answer would be, to contact the sequencing facility and bioinformatic service at WEHI.

from tidybulk.

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