Comments (3)
This was solved with the new versions of tidybulk and SummarizedExperiment soon to be merged.
I hope your PhD is going well.
from tidybulk.
@stemangiola
Hi Stefano,
Thanks! It's going ok. Hope you had a wonderful trip to Italy!
We just got our scRNAseq data. I'm trying to convert the bcl files into fastq files but found that the SampleSheet.csv file:
(for example: https://github.com/YichaoOU/HemTools/blob/master/docs/gallery/SampleSheet.csv )
that contains information such as the adapter, flow cell lane, etc are missing. Can I ask if I should ask WEHI sequencing people for this file or if we could create this file myself in order to use bcl2fastq?
Here is the data folder given by WEHI sequencing staff:
Kind regards,
Jian
from tidybulk.
Hello Jian,
better we bring this conversation to email, as it is not really about tidybulk.
My answer would be, to contact the sequencing facility and bioinformatic service at WEHI.
from tidybulk.
Related Issues (20)
- pivot_sample and pivot_transcript convert .sample and .feature to sample and feature HOT 2
- Allow logFC shrinkage in DESeq HOT 4
- Normalization method (scaling) HOT 9
- for SE save internals within the metadata rather than attributes.
- github action mac fail HOT 1
- DEG Analysis Between Single Group with Multiple Groups HOT 6
- "Proportion of variance explained" values on reduce_dimensions() output HOT 1
- github actions give preflight error for the whole tidy stack HOT 1
- ERROR; return code from pthread_create() is 22 HOT 3
- pathway analysis using tidybulk HOT 8
- Design and contrasts for tidybulk test differential abundance function HOT 9
- Remove batch effect in test_differential_abundance
- Warning in CHECK, mistery
- test
- Improve the documentation for `tidybulk` HOT 29
- turn pseudobulk from scRNAseq with logCPM value back to a seurat object with counts value? HOT 2
- `tidybulk` Remove hard dependency for `DESeq2` HOT 12
- in `test_gene_rank` check if any feature is NA, because pivot_feature will fail
- the attribute "internals" $ tt_columns abundance_scaled, carries the environment with it inflating the RDS file size
- Prepare for upcoming Seurat v5 release
Recommend Projects
-
React
A declarative, efficient, and flexible JavaScript library for building user interfaces.
-
Vue.js
🖖 Vue.js is a progressive, incrementally-adoptable JavaScript framework for building UI on the web.
-
Typescript
TypeScript is a superset of JavaScript that compiles to clean JavaScript output.
-
TensorFlow
An Open Source Machine Learning Framework for Everyone
-
Django
The Web framework for perfectionists with deadlines.
-
Laravel
A PHP framework for web artisans
-
D3
Bring data to life with SVG, Canvas and HTML. 📊📈🎉
-
Recommend Topics
-
javascript
JavaScript (JS) is a lightweight interpreted programming language with first-class functions.
-
web
Some thing interesting about web. New door for the world.
-
server
A server is a program made to process requests and deliver data to clients.
-
Machine learning
Machine learning is a way of modeling and interpreting data that allows a piece of software to respond intelligently.
-
Visualization
Some thing interesting about visualization, use data art
-
Game
Some thing interesting about game, make everyone happy.
Recommend Org
-
Facebook
We are working to build community through open source technology. NB: members must have two-factor auth.
-
Microsoft
Open source projects and samples from Microsoft.
-
Google
Google ❤️ Open Source for everyone.
-
Alibaba
Alibaba Open Source for everyone
-
D3
Data-Driven Documents codes.
-
Tencent
China tencent open source team.
from tidybulk.