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bam2fastx's Introduction

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BAM2fastx tools

The BAM2fastx tools allow conversion of PacBio BAM files into gzipped fasta and fastq files, including demultiplexing of barcoded data.

DEPENDENCIES

Please install Boost and zlib.

INSTALL

  • Download source from github

      git clone https://github.com/PacificBiosciences/bam2fastx && cd bam2fastx
    
  • Sync your code with the latest git code base:

      git pull --rebase origin master && git submodule update --init
    
  • Create build directory

      mkdir build && cd build
    
  • Invoke cmake with Boost and zlib as system libraries

      cmake ..
    
  • Or with user-specific paths

      cmake -DBoost_INCLUDE_DIRS=/path/to/boost/include -DZLIB_INCLUDE_DIRS=/path/to/zlib/include -DZLIB_LIBRARIES=/path/to/zlib/lib ..
    
  • Build

      make
    

USAGE

Both tools have an identical interface and take BAM and/or DataSet files as input. Examples:

    bam2fasta -o projectName m54008_160330_053509.subreads.bam
    bam2fastq -o myEcoliRuns m54008_160330_053509.subreads.bam m54008_160331_235636.subreads.bam
    bam2fasta -o myHumanGenome m54012_160401_000001.subreadset.xml

HELP

Futher help can be requested with -h. Issues? Problem? Please create a github issue.

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