Comments (8)
link in README here to https://kablag.github.io/RDML/ seems dead and I cannot find it anywhere else
now it is here https://pcruniversum.github.io/RDML/
Problem is, if I'am importing a file exported from CFX Manager, I get the fdata with no error, while if the file was exported from CFX Maestro, the raw_data$GetFData() step always says:
I don't have CFX Maestro now. So please attach your rdml and pcrd files.
Also, I noticed that CFX Manager failed to open results produced from CFX96Touch & CFX Maestro, while CFX Maestro was able to open files from
CFX96Touch & CFX Maestro and CFX Connect & CFX Manager, although they were all xxxx.pcrd files (maybe due to software compatibility issues?)
CFX Maestro somehow change file from CFX Manager that it can't be open in CFX Manager after saving. That's why I don't use CFX Maestro anymore… Small improvements in statistical analysis don't worth it.
from rdml.
now it is here https://pcruniversum.github.io/RDML/
No, I mean the link to Manual in README is dead, https://kablag.github.io/RDML/. The Manual Section at https://pcruniversum.github.io/RDML/ also points to https://kablag.github.io/RDML/.
I don't have CFX Maestro now. So please attach your rdml and pcrd files.
Here is a rmdl v1.1 and pcrd file from Maestro:
It was one of my runs performed by CFX96Touch, result was produced from Maestro and rdml exported from Maestro too. The pcrd file cannot be opened by CFX Manager and the GetFData()
step failed with errors mentioned above.
CFX Maestro somehow change file from CFX Manager that it can't be open in CFX Manager after saving. That's why I don't use CFX Maestro anymore… Small improvements in statistical analysis don't worth it.
That's something I cannot decide as the two instruments are owned by the lab and I don't get to installed/uninstall softwares on the computers attched to them.
Thanks.
from rdml.
No, I mean the link to Manual in README is dead, https://kablag.github.io/RDML/. The Manual Section at https://pcruniversum.github.io/RDML/ also points to https://kablag.github.io/RDML/.
Corrected.
It was one of my runs performed by CFX96Touch, result was produced from Maestro and rdml exported from Maestro too. The pcrd file cannot be opened by CFX Manager and the GetFData() step failed with errors mentioned above.
I looked at RDML file generated by Maestro and it does not contain any fluorescence points at all:
<react id="56"><sample id="group4" /><data><tar id="Actin" /><cq>16.1183718203916</cq></data></react>
only Cq. So I can't correct this. Maybe Maestro contain some export options? Or try to export in different RDML format versions and send to me generated files.
from rdml.
I looked at RDML file generated by Maestro and it does not contain any fluorescence points at all:
<react id="56"><sample id="group4" /><data><tar id="Actin" /><cq>16.1183718203916</cq></data></react>
only Cq. So I can't correct this. Maybe Maestro contain some export options? Or try to export in different RDML format versions and send to me generated files.
Unfortunately there aren't any options in setting regarding exporting to RDML except to v1.0 or v1.1. Anyway I export the same file to RDML v1.0 and here it is(it's actually not a zip file, just change .zip
to .rdml
):
In Maestro, if you try the "Custom Export" there will be options regarding what to include in the output files, but RDML is not supported in custom export. Anyway, I chose to export anything I see into a XML file and here it is(again it's actually not a zip file, just remove .zip
):
from rdml.
Anyway I export the same file to RDML v1.0 and here it is
It does not contain fluorescence points too…
Maybe you have to make some PCR analysis inside Maestro, then save file and export? For example ABI7500 adds data after analysis.
Another option is ask Biorad about this issue.
from rdml.
It does not contain fluorescence points too…
Maybe you have to make some PCR analysis inside Maestro, then save file and export? For example ABI7500 adds data after analysis.
Well, I've already done some analysis, this is a plot for gene2 expression relative to NC group:
Another option is ask Biorad about this issue.
I'll try to contact BioRad for this.
Thank you @kablag for your paitence and advice.
from rdml.
I'll try to contact BioRad for this.
keep me in touch please if they answer you
from rdml.
@kablag I will. But they don't seem to reply to my email feedbacks.
Sent from my ONEPLUS A3010 using FastHub
from rdml.
Related Issues (20)
- New documentation HOT 2
- core speedup
- summary speedup
- Can't Install in R 3.2 Mac 10.10.3 HOT 1
- Getting documentationError: id is not a 'idType' or length > 1 HOT 5
- RDML$new fails with multidye file HOT 4
- Error in : Some prefixes [d2, d3] don't already exist. HOT 5
- Validation of RDML files created by RDML package fails HOT 1
- error log messages when loading files on the RDMLedit web-server HOT 2
- Error 'File not found' when trying to export from RDMLedit web-server HOT 3
- Issues running RDMLedit() locally from Rstudio HOT 2
- Merge RDML files by exp.id HOT 1
- RDML$new assertion error NA HOT 2
- rdmlEdit() problem with extendShinyjs: `functions` argument must be provided. HOT 1
- The link to the web server indicated in the README is broken
- Warn about `RDML::rdmlEdit()` dependence on SUGGESTS packages, e.g. shinythemes, etc.
- shinyjs: extendShinyjs: `functions` argument must be provided. See the documentation for `?extendShinyjs` for more details.
- RDML$new assertion error NA HOT 3
- loading file results in "Warning: Error in <-: attempt to set an attribute on NULL" HOT 4
Recommend Projects
-
React
A declarative, efficient, and flexible JavaScript library for building user interfaces.
-
Vue.js
🖖 Vue.js is a progressive, incrementally-adoptable JavaScript framework for building UI on the web.
-
Typescript
TypeScript is a superset of JavaScript that compiles to clean JavaScript output.
-
TensorFlow
An Open Source Machine Learning Framework for Everyone
-
Django
The Web framework for perfectionists with deadlines.
-
Laravel
A PHP framework for web artisans
-
D3
Bring data to life with SVG, Canvas and HTML. 📊📈🎉
-
Recommend Topics
-
javascript
JavaScript (JS) is a lightweight interpreted programming language with first-class functions.
-
web
Some thing interesting about web. New door for the world.
-
server
A server is a program made to process requests and deliver data to clients.
-
Machine learning
Machine learning is a way of modeling and interpreting data that allows a piece of software to respond intelligently.
-
Visualization
Some thing interesting about visualization, use data art
-
Game
Some thing interesting about game, make everyone happy.
Recommend Org
-
Facebook
We are working to build community through open source technology. NB: members must have two-factor auth.
-
Microsoft
Open source projects and samples from Microsoft.
-
Google
Google ❤️ Open Source for everyone.
-
Alibaba
Alibaba Open Source for everyone
-
D3
Data-Driven Documents codes.
-
Tencent
China tencent open source team.
from rdml.