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View Code? Open in Web Editor NEWTool for writing matrix of AUCs of indexed BAMs in regions specified by BED
License: MIT License
Tool for writing matrix of AUCs of indexed BAMs in regions specified by BED
License: MIT License
I'm using sorted BAM files that have numeric chromosome names (no "chr" prefix). There seems to be an issue with quantification. If I remove "chr" from your degradation BED file chromosome names, I get 0's for quantification of regions on chromosomes 10-19.
If I sort the BED file so that numeric chromosomes are in numeric order (to match BAM files), I get the following error I think from wiggleTools:
"Position 10:714133 is before 9:140899137"
even though the BED file looks like
9 140049063 140050870 . 0 .
9 140791269 140791603 . 0 .
9 140833366 140833967 . 0 .
9 140899136 140899908 . 0 .
10 714132 714671 . 0 .
10 7830132 7830226 . 0 .
10 51565182 51565296 . 0 .
10 51580555 51580696 . 0 .
10 51632928 51633033 . 0 .
10 60028911 60029064 . 0 .
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