methylcytosine calling (5mC calling) from BS-seq. Bycom can do methylcytosine calling from BS-seq (WGBS and RRBS), and either unmapped reads (FASTQ) or mapped reads (SAM/BAM) could be permitted for the input data. Certain SNPs (C>A/G) can also be selected in the output.
- There's no softwares or methods identify methylcytosines considering the cell heterozygosis caused by multicellular sequencing. Bycom introduced it along with the sequencing errors and unconverson rate based on the Bayesian model.
- Several parameters in Bycom could be set as what the users want to, such as depth threshold, quality control value, conversion rate, processor number.
- Bycom do the mapping based on BSMAP, and provide the parameters using in the alignment.