This is a basic python script for the web-based tool (I call it NetDNA-1.0). It can easily run on Streamlit, and generates basic biological data from the query DNA sequence.

• Length of the Sequence
• Count of Non-bases(if any)
• Nucleotide Base-Count & its visualization
• GC Content(%)
• Melting Temperature(Tm)
• Reverse Complement
• Transcribed Sequence
• Translated Sequence

Please refer to the following guide - Streamlit Documentation to configure Streamlit.

Once streamlit is configured, download the above files and place it in a directory.

Go to the above directory and run the script using the command:

streamlit run NetDNA-1.0.py

This will open the tool in the browser as a web-based tool!

Note: Calculation of Melting Temperature(Tm) is just an example, because Tm is usually calculated for primers (typically 18-30 bp in length) and not the whole DNA sequence as in the above example.