Comments (3)
lh3
commented on July 21, 2024
Could you show the violating line before and after "-VQ64"?
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WinterLi1993
commented on July 21, 2024
I think If I convert to 64phred ,should not appear "-29 quality" , but I use fastQc tell something wrong happend on fastq phred.
seqtk seq -V -Q 64 $1.fq > $1.s.fq
seqtk seq -V -Q 64 $2.fq > $2.s.fq
"fastq_qc_stat -a $1.fq $2.fq -f R02_N -q 45 -Q 64
Error Information： “FATAL ERROR (file fastq_qc_stat.c line 80): quality (-29) not in [0, 45]”
seqtk is work ??
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lh3
commented on July 21, 2024
-VQ64 converts Q64 quality to Q33 quality, not the other way around.
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