Comments (2)
@danielecook which command did you use to demux?
Usage: seqtk <command> <arguments>
Version: 1.0-r82-dirty
Command: seq common transformation of FASTA/Q
comp get the nucleotide composition of FASTA/Q
sample subsample sequences
subseq extract subsequences from FASTA/Q
fqchk fastq QC (base/quality summary)
mergepe interleave two PE FASTA/Q files
trimfq trim FASTQ using the Phred algorithm
hety regional heterozygosity
gc identify high- or low-GC regions
mutfa point mutate FASTA at specified positions
mergefa merge two FASTA/Q files
dropse drop unpaired from interleaved PE FASTA/Q
rename rename sequence names
randbase choose a random base from hets
cutN cut sequence at long N
listhet extract the position of each het
from seqtk.
Haha - I have no idea. This was a while ago - so I either confused this with another tool or I used this to remove the indices after demultiplexing. I've since switched to using trimmomatic, however.
from seqtk.
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