Comments (1)
Using exactly the command line you specified (including the -Xmx512m VM argument), I get a java.lang.OutOfMemoryError (using OpenJDK build 11.0.1+13-LTS).
If I remove the memory limit, I do not get any errors and the expected output file.
So I would assume that either the memory limit has some side effect on your system (OS, Java version), or the file download of the Bigwig file has been corrupted. For the latter, you could check the MD5sum of your local file. According to ENCODE, this should be 2d4042c08a88bc6bb72e9a73fa7d30ea, and I got the same for my local file.
The error message you reported is from the IGV BBFileReader (see https://github.com/lindenb/bigwig/blob/master/src/org/broad/igv/bbfile/BBFileReader.java), which we use internally for reading Bigwig files, and should indicate a corrupted header of the Bigwig file.
from jstacs.
Related Issues (20)
- usage about the parameter of weight (w) HOT 4
- amino acid J found in input HOT 1
- GeMoMa Extractor Error: There are gene annotations on chromosomes/contigs with missing reference sequence HOT 2
- java.lang.IllegalArgumentException: At least two sequences with the same ID but different sequence: HOT 5
- Using RNA-seq from closely-related organism HOT 2
- GeMoMa expected runtime HOT 2
- How does GeMoMa treat masked nucleotides? HOT 1
- Problem when adding external evidence HOT 5
- cdsParts=true, but the ID (gene-si: )seems to be no CDS part HOT 1
- AnnotationFinalizer for renaming genes and transcripts HOT 2
- Exception in thread "main" java.lang.NullPointerException HOT 2
- Why does GeMoma annotationfianlizer remove the exon features ? HOT 1
- Could not open GeMoMa_temp/GeMoMaPipeline-9364982901453846136/mmseqsdb_h.index.5 for writing! HOT 2
- java.lang.OutOfMemoryError: Java heap space when using GeMoma HOT 21
- java.lang.InterruptedException HOT 1
- Use GeMoma with protein as reference, the result showed no predicted_proteins.fasta and final_annotation.gff HOT 13
- Result filtering HOT 4
- GeMoMa error HOT 8
- No gene model was extracted from the references. HOT 3
- GeMoMa gives me multiple genes with exact same coordinates HOT 6
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from jstacs.