Comments (1)
Hi Rich,
Thanks for the report.
I have downloaded the genome and the annotation from https://www.ncbi.nlm.nih.gov/genome/?term=txid7955[orgn] and ran the Extractor:
java -jar GeMoMa-1.9.jar CLI Extractor g=GCF_000002035.6_GRCz11_genomic.fna.gz a=GCF_000002035.6_GRCz11_genomic.gff.gz
which worked fine. So, you probably ran the complete pipeline and got the error from annother module. I had a closer look at the error message and wondered about the gene ID which is given as gene-si:
(with a blank as last character an not further characters). Hence, I checked the annotation and found the several IDs have the pattern gene-si: .*
. This might be a problem. The Extractor create FASTA file for the CDS-parts with the ID as prefix and the module GeMoMa cuts the part after the space while reading, as whitespace is often used as delimiter in FASTA. Hence, it will throw an error.
This error has been reported here: #39
For the moment, you can replace the whitespaces in your annotation mannually and run GeMoMa as before.
I have already implemented a solution with an additional parameter that will be released with the next version.
I hope that helps.
best regards, Jens
from jstacs.
Related Issues (20)
- amino acid J found in input HOT 1
- GeMoMa Extractor Error: There are gene annotations on chromosomes/contigs with missing reference sequence HOT 2
- java.lang.IllegalArgumentException: At least two sequences with the same ID but different sequence: HOT 5
- Using RNA-seq from closely-related organism HOT 2
- GeMoMa expected runtime HOT 2
- How does GeMoMa treat masked nucleotides? HOT 1
- Problem when adding external evidence HOT 5
- AnnotationFinalizer for renaming genes and transcripts HOT 2
- Exception in thread "main" java.lang.NullPointerException HOT 2
- Why does GeMoma annotationfianlizer remove the exon features ? HOT 1
- Could not open GeMoMa_temp/GeMoMaPipeline-9364982901453846136/mmseqsdb_h.index.5 for writing! HOT 2
- java.lang.OutOfMemoryError: Java heap space when using GeMoma HOT 21
- java.lang.InterruptedException HOT 1
- Use GeMoma with protein as reference, the result showed no predicted_proteins.fasta and final_annotation.gff HOT 13
- Result filtering HOT 4
- GeMoMa error HOT 8
- No gene model was extracted from the references. HOT 3
- GeMoMa gives me multiple genes with exact same coordinates HOT 6
- Issues with CLI Analzyer HOT 5
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