bigcsv allows for easy calculation of csv transposes, even when the csv is much too large to fit in memory.

If data is purely numeric, it is much more efficient to store in in h5ad (readable by AnnData), which uses the amazing HDF5 format under-the-hood.

To install, run pip install bigcsv

All operations are method of the BigCSV class, which contains metadata information used to do all calculations.

from bigcsv import BigCSV

obj = BigCSV(
    file='massive_dataset.csv',
    chunksize=400, # Number of rows to read in at each iteration
    # leave as default
    # insep=',', 
    # outsep=',',
    # chunksize=400, 
    # save_chunks=False,
    # quiet=False,
)

obj.to_h5ad(outfile='converted.h5ad')

# Or maybe we want to keep as csv, but transpose it (in the case of non-numerical data)
obj.transpose(outfile='dataset_T.csv')

1. bigcsv.BigCSV Class containing methods for manipulating csvs.

file: Path to input file
outfile: Path to output file (transposed input file)
insep=',': Input separator for delimited file, by default is , outsep=',': Output separator for delimited file (in the case of csv --> csv operations) chunksize=400: Number of lines per iteration
save_chunks: To save intermediate chunks or not chunkfolder=None: Optional, Path to chunkfolder
quiet=False: Boolean indicating whether to print progress or not

2. bigcsv.BigCSV.transpose_csv

outfile=None: Ouput file to write to, or if specified in initialization, writes to that file name

3. bigcsv.BigCSV.to_h5ad

outfile=None: Ouput file to write to, or if specified in initialization, writes to that file name sparsify: bool=False: Sparsify rows in h5 matrix compression: str='infer': Compression format of input csv, if compressed. Probably just leave to infer unless the filename is weird. lines: int=None: Number of lines in the file. If you know a priori, this saves some time. Also cannot be calculated for compressed files. dtype: Any=None: dtype of entries of input matrix index_col: str=None: Column of input csv to use as index, if any. index: bool=True: Save index when converting to h5ad.