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HPC Automated Pipeline Processing Yotta Neurons

This library aim to provide integration of different libraries used for neuroscience with HPC facilities.

Currently the library is organized in 3 levels:

  • Unified install and env system for all third part applications used
  • Stand alone application wrappers that can be invoked by terminal or imported as a library
  • Automated execution functions using ALCF Balsam

Getting Started

These instructions will get you a copy of the project up and running on your local machine for development and testing purposes. See deployment for notes on how to deploy the project on a live system.

Prerequisites

What things you need to install the software and how to install them

  • Neuroglancer
  • CloudVolume
  • Knossos Utils
  • ffn
  • etc

Building your preamble at Cooley HPC.

A step by step series of examples that tell you how to get a development env running

Say what the step will be

Building your preamble at Theta HPC.

A step by step series of examples that tell you how to get a development env running

List of Macros in workflow form

Data Acquisition

  • Generate dataset Preview
  • Generate dataset quality check
  • Generate bad-image list

Tile Image Processing

  • Correct each image individually
  • Correct brightness individually

Slice Montage (X-Y dimension)

  • Pairwise aligment
  • Global Optimzation
  • Stitching

Alignment (Z dimension)

  • Pairwise aligment
  • Global Optimzation
  • Cube rendering

Visualization

  • Cube cropping (removing borders)
  • Cube neuroglancing
  • Inset cropping

Training

  • Inset Manual annotation
  • FFN compute_partitions
  • FFN build_coordinates
  • FFN train

Inference

  • Splice data
  • Inference
  • Merge

Final Visualization

  • Precompute data
  • Precompute Labels
  • Generate Mesh

Other Macros

  • Knossos injection/extraction
  • webKnossos injection/extraction

Versioning

We use SemVer for versioning. For the versions available, see the tags on this repository.

Authors

  • Rafael Vescovi - Initial work
  • Hanyu Li - Initial work
  • Murat Keceli - Initial work
  • Jeff Kinnisson - Initial work
  • Bobby Kasthuri - Initial work
  • Nicola Ferrier - Initial work
  • Rafael Vescovi - Initial work

See also the list of contributors who participated in this project.

License

This project is licensed under the MIT License - see the LICENSE.md file for details

Acknowledgments

  • Argonne National Laboratories
  • University of Chicago
  • Harvard University
  • Princeton University
  • Google

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