gregorkrz / dna-melt-analysis Goto Github PK

This software helps to automate analysis of UV melting curves. It currently has the following functionalities:

• Easy pooling of data from different files and querying data by name
• Automatic or manual baseline correction
• Primer melting temperature (Tm) determination
• Rendering of Absorbance and Fraction Folded plots, with custom experiment labels, colors and markers
• Export to Excel workbook

pip3 install -r requirements.txt

The csv data should be in the following format:

Sample1Name,,Sample2Name,,Sample3Name,
Temperature,Abs,Temperature,Abs,Temperature,Abs
10.0,0.45,10.0,0.55,10.0,0.45
...

See example data.

The following command will run the example configuration: python3 src/main.py config.txt.example

When prompted (e. g. when a plotting operation was selected) to select experiments, a list of experiments prefixed with their IDs will appear. The following select queries are supported:

• comma separated list of experiment IDs to plot
• an ID range to plot, e. g. 0-5
• searching by experiment name: query f=text1,text2 will select all experiments that contain string text1 AND string text2