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Geng Lee's Projects

bam-to-junction-bed icon bam-to-junction-bed

Uses SAMTools, Python 2.7 and PySam to extract either junctions or exon read counts for known and novel junctions and exons. Requires an indexed BAM file with genomic coordinates

bcbio-nextgen icon bcbio-nextgen

Validated, scalable, community developed variant calling, RNA-seq and small RNA analysis

be-smart icon be-smart

A deep learning tool for CRISPR base editing efficiency prediction

be_predict_bystander icon be_predict_bystander

Deep conditional autoregressive models for genome editing sequence-to-sequence problems

beautiful-jekyll icon beautiful-jekyll

✨ Build a beautiful and simple website in literally minutes. Demo at https://beautifuljekyll.com

belib_processing icon belib_processing

Code for processing data from base editing screens performed with the libraries first described in Arbab, Shen, et al., Cell (2020). Contains code used for Neugebauer et al., Nat. Biotechnol. (2022)

benchmarking_variant_callers icon benchmarking_variant_callers

In this study, we perform systematic comparative analysis of seven widely-used SNV-calling methods, including SAMtools, the GATK Best Practices pipeline, CTAT, FreeBayes, MuTect2, Strelka2 and VarScan2, on both simulated and real single-cell RNA-seq datasets. We evaluate the performances of these tools in different read depths, genomic contexts, functional regions and variant allele frequencies.

besensor icon besensor

Files related to base editing sensor data and BE-SCAN Shiny app

biancur-cellmetabolism-2020 icon biancur-cellmetabolism-2020

Corresponding code and figures for publication of Biancur DE, Kapner KS, Yamamoto K, Banh RS, Neggers JE, Sohn ASW, Wu W, Manguso R, Brown A, Root D, Aguirre AJ, Kimmelman AC. Functional Genomics Identifies Metabolic Vulnerabilities in Pancreatic Cancer. Cell Metabolism, 2020, In Press.

breat-cancer-screening-classification-and-evaluation-using-confusion-matrix icon breat-cancer-screening-classification-and-evaluation-using-confusion-matrix

This project was made for a client on PeoplePerHour. The data involves screening different columns and then plotting out confusion matrices using the Seaborn Library and HeatMaps. The True positives, False positives, True negatives, False negatives are calculated and then used to calculate specificity and sensitivity.

brspdata icon brspdata

Allen Institute BrainSpan Exon and Gene Expression Values

capanno icon capanno

cwl/wdl/sm/nf files, tool and workflow metadata, and input specifications.

cas-analyzer icon cas-analyzer

Full JavaScript-based assessment tool for targeted sequencing data of genome-edited samples.

cell-state-atlas-2022 icon cell-state-atlas-2022

Code for a multimodal atlas of healthy and injured cell states and niches in the human kidney

clam icon clam

Data Efficient & Weakly Supervised Computational Pathology on Whole Slide Images

cld icon cld

A software for the multispecies design of CRISPR/Cas9 libraries

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