CirSeq is a new HTS technique of RNA virus populations. The technique revolves around circularisation of fragmented viral RNAs. Upon sequencing these circularised fragments, a consensus sequence of the initial RNA template is derived.
High-Throughput Sequencing (CirSeq)
- Studying the genetic changes underlying the evolution of virus populations.
- Define the mutation rates of poliovirus and uncover the mutation landscape of the population
The statistical significance of mutations detected was determined by a one-sided binomial test in R using the average estimated error probability at each genome position as the null probability of success.
@Jiayi-Wang-Joey
@nhorozov
@garcia-mc
[Acevedo, A., Brodsky, L. & Andino, R. Mutational and fitness landscapes of an RNA virus revealed through population sequencing. Nature 505, 686โ690 (2014).] (https://doi.org/10.1038/nature12861)