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Epitope Prediction Workflow

Download Dengue Sequence

Initial Steps

  • Open File with MS Word
  • Check Length

Epitope Prediction: MHC-I Binding

  • Step-1: Go to https://www.iedb.org/
  • Step-2: Epitope Analysis Resource > T Cell Epitope Prediction > MCH-I Binding
  • Step-3: Enter protein sequence(s) in FASTA format or as whitespace-separated sequences or Or select file containing sequence(s)
  • Step-4: Show only frequently occuring alleles: Select MHC allele(s) > Allele > HLA-A*01:01 > Length > 9
  • Step-5: Repeat: Show only frequently occuring alleles: Select MHC allele(s) > Allele > HLA-A*01:01 > Length > 9
  • Step-6: Submit

Epitope Prediction: MHC-II Binding

  • Step-1: Go to https://www.iedb.org/
  • Step-2: Epitope Analysis Resource > T Cell Epitope Prediction > MCH-II Binding
  • Step-3: Enter protein sequence(s) in FASTA format or as whitespace-separated sequences or Or select file containing sequence(s)
  • Step-4: Show only frequently occuring alleles: Select MHC allele(s) > Allele > DRB1*01:01 > Length > 15
  • Step-5: Repeat: Show only frequently occuring alleles: Select MHC allele(s) > Allele > DRB1*01:03 > Length > 15
  • Step-6: Submit

Team

coinfection's People

Contributors

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Watchers

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