Failing to demultiplex mock-3 about mockrobiota HOT 3 CLOSED

Thanks for raising this issue, @benjjneb . I got some advice from @wasade , who has processed mock-3 with qiime1 more recently (I have been using qiime2 without issue on this dataset). He notes that:

it looks like we needed to rc both mapping and the index read
and specify --phred_offset 33
it looks like we also needed to use the simplified headers, where the " 1:N:0:1" bits were removed

@benjjneb if you can confirm that this works for you, and provide a working qiime1 command to me, I will correct this in the mock-3 README and close this issue. Thanks!

from mockrobiota.

Thanks, can confirm the following worked:

split_libraries_fastq.py -i mock-forward-read.fastq -m sample-metadata.tsv -o out --store_demultiplexed_fastq -b mock-index-read.fastq --rev_comp_mapping_barcodes --rev_comp_barcode --phred_offset 33

from mockrobiota.

Thanks @benjjneb and @wasade for testing this! The corrected readme is fixed with #62 .

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