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a-counterdown-timer

设置事件名称和发生时间等参数，无边框置顶在特定位置

alist

阿里云盘列表

aliyundrive-webdav

阿里云盘 WebDAV 服务

anti-screenshot

一个强制截屏的xposed module

assemblies-of-putative-sars-cov2-spike-encoding-mrna-sequences-for-vaccines-bnt-162b2-and-mrna-1273

RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.

auto-gpt

An experimental open-source attempt to make GPT-4 fully autonomous.

autoapis

超级版，多账号，备用模式，随机api

autoapisecret-e5

加密版，应用id/机密不再可见

autorclone

A project that allows you copy large folders to Shared Drives.

baidu-cloud-download-acceleration

百度云网盘下载加速器

baidu-netdisk-downloaderx

⚡️ 一款图形界面的百度网盘不限速下载器，支持 Windows、Linux 和 Mac。已于 2020 年 4 月 15 日正式停用，源码仅用于程序员交流学习，细节请查看：关于停用 BND 的说明 https://hacpai.com/article/1586956316578

baidu-netdisk-downloaderx-1

baidupcs-batch-upload

使用baidupcs-go,百度网盘批量上传文件

baidupcs-go

百度网盘客户端 - Go语言编写

baidupcs-web

自用BUG修复

baiduwp

PanDownload Web, built with CloudFlare Workers

baiduyun

🖖油猴脚本 一个脚本搞定百度网盘下载

bbr2

Google BBRv2 Kernel

better-cloudflare-ip

查找适合自己当前网络环境的优选cloudflare anycast IP

bot

bypass-paywalls-chrome

Bypass Paywalls web browser extension for Chrome and Firefox.

caddy

Fast, cross-platform HTTP/2 web server with automatic HTTPS

ccaa

Linux一键安装Aria2 + AriaNg + FileBrowse实现离线下载、文件管理。

ccxt

A JavaScript / Python / PHP cryptocurrency trading library with support for more than 100 bitcoin/altcoin exchanges

cfwarp-pro

仅支持"内核集成"模式的WARP多功能脚本（支持ARM/X86）：纯IPV4、纯IPV6、双栈IPV4+IPV6 各种VPS都可用。目前已测试通过oracle（甲骨文云），gpc（谷歌云），buyvm，racknerd，virmach，vultr，aws（亚马逊云），azure（微软云），bandwagonhost（搬瓦工）……欢迎大家补充反馈！

chatgpt-telegram-workers

Deploy your own Telegram ChatGPT bot on Cloudflare Workers with ease.

cleanremotes

cloudflare

cloudflarespeedtest

🌩 测试 Cloudflare CDN 延迟和速度，获取最快 IP (IPv4+IPv6)！

cobinhood_api

An API wrapper for Cobinhood written in Python