Comments (1)
@michaelbarton wow - thanks for letting me know. Looking at their latest manual I see
Do not use -s options for single-read libraries, since it
specifies unpaired reads for the first paired-end library.
In a way, these merged reads are like orphan PE reads. In many cases they fully overlap so are no different to just providing R1 and not R2.
But the Spades team know their software and I am excited to change this in the hope it improves results even further!
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Related Issues (20)
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