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rnakato avatar rnakato commented on July 27, 2024

Hi nepsygirl,
Did you add the backslashes (\) without line break? What if you remove the backslashes?
In addition, your sample lines (-i option) have spaces before and after "," , and the sample labels look specified at the "input" position. Try to change them like this: -i $dir/GSM3752858_0-7A-H3K27ac.100.bw,,GSM3752858_0-7A-H3K27ac

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nepsygirl avatar nepsygirl commented on July 27, 2024

Hi rnakato,

Thank You for the comment, may be i was doing something wrong. So I tried your tutorial first, downloaded all files and tried to create pdf. So far I am unable to create the file.

`(base) [root@localhost Probe]# drompa+ PC_SHARP \

-i $dir/H3K4me3.100.bw, $dir/Input.100.bw, H3K4me3
-i $dir/H3K27me3.100.bw, $dir/Input.100.bw, H3K27me3
-i $dir/H3K36me3.100.bw, $dir/Input.100.bw, H3K36me3
-o pdf1 -g $gene --gt $gt
--lpp 2 --showitag 2
--chr 1
Segmentation fault (Speicherabzug geschrieben)`

(base) [root@localhost Probe]# drompa+ PC_SHARP -i $dir/H3K4me3.100.bw, $dir/Input.100.bw, H3K4me3 -i $dir/H3K27me3.100.bw, $dir/Input.100.bw, H3K27me3 -i $dir/H3K36me3.100.bw, $dir/Input.100.bw, H3K36me3 -o pdf1 -g $gene --gt $gt --lpp 2 --showitag 2 --chr 1
Segmentation fault (Speicherabzug geschrieben)

`

(base) [root@localhost Probe]# drompa+ PC_SHARP \

-i $dir/H3K4me3.100.bw, $dir/Input.100.bw, H3K4me3
-i $dir/H3K27me3.100.bw, $dir/Input.100.bw, H3K27me3
-i $dir/H3K36me3.100.bw, $dir/Input.100.bw, H3K36me3
-o pdf1 -g $gene --gt $gt
--lpp 2 --showitag 2
--chr 1
the argument ('2--chr') for option '--showitag' is invalid`

For the top one I added as you have it in tutorial, problem was segmentation fault, didn't understand what that was, couldn't solve it, I tried in root too :, the lower one I tried to remove spaces before '' which results in syntax error.

I think the top one is some how correct but cannot find how to solve this problem.
Please let know if you have some suggestions.

Thanks a lot

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nepsygirl avatar nepsygirl commented on July 27, 2024

Hi rnakato,

Please Reply!

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rnakato avatar rnakato commented on July 27, 2024

Why do you modify the commnad? Please try:

drompa+ PC_SHARP \
         -i $dir/H3K4me3.100.bw,$dir/Input.100.bw,H3K4me3 \
         -i $dir/H3K27me3.100.bw,$dir/Input.100.bw,H3K27me3 \
         -i $dir/H3K36me3.100.bw,$dir/Input.100.bw,H3K36me3 \
         -o drompa1 -g refFlat.txt --gt genometable.txt \
         --lpp 2 --showitag 2 --chr 1

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