Comments (1)
samuelecancellieri
commented on June 25, 2024
Hi,
thanks for using the tool.
Regarding the PAM mismatches, we do not support mismatches in the PAM, if you search with NNN and generate the index with it, the tool will find any possible sequence but not reporting mismatches in the PAM sequence.
Regarding the DNA+RNA bulges, no, we support only type of bulge in a specific target, you can have the same target reported with both in different alignments but not both in the same target.
Thanks a lot for using the tool, if you have any more question, let me know.
Best
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Related Issues (14)
- Early stopping HOT 2
- -score question? HOT 1
- how to infer cutting sites from the result?
- how was the annotation derived? HOT 2
- Crash when running on non-human genome
- generate-report Syntax-Warning
- A question about index-genome HOT 2
- Python 3 incompatibilities
- PAM NNGRRT HOT 1
- how to generate annotation file for other species HOT 4
- how to recognize the original input guide sequences from crispritz search results HOT 1
- how to understand the radar chart? HOT 2
- Calculating Start and End Positions HOT 3
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