Comments (6)
Yeah, this is in my to-do-list. I'll export a vcf file format. For the current output, each column representation could be find here: https://github.com/parklab/xTea_paper/tree/main/run_tools/xTea/HG002.
There is a intermediate file called candidate_list_from_clip.txt
has the number of clipped reads (third column), but I didn't count the mapped...
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Thanks for the pipeline, it is a very useful tool.
Follow up on the xtea_long output: why do the SVA insertion positions often start from a negative value?
Like the 2nd line here: https://github.com/parklab/xTea_paper/blob/main/run_tools/xTea/HG002/HG002_hg38_Nanopore_xTea_SVA.txt
chr6 138775846 SVA None -1411:1274:+ None
Thank you very much!
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negative value
indicates the insertion is started/ended from the flanking region (likely to be transduction). But maybe also the reported annotation is incorrect.
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Thanks for your swift reply! Are there ways to infer the correct consensus position for SVA?
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It's not straightforward as the reference SVA annotation is fragmented and inaccurate (because of the tandem repeats expansion). For a simple way, just consider position 0 as the start position on the consensus, but it may be inaccurate.
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Thank you, a useful tool for analyzing TEs.
Can xtea_long now generate vcf file directly? I find my output is still classified_results*.txt. I don't know if I made a mistake.
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Related Issues (20)
- x_TEA_main.py: error: no such option: --bamsnap HOT 2
- Very long length of L1 insertion HOT 1
- Long_reads: File detailing which reads show transductions of deletions HOT 1
- temp files burst out HOT 2
- Possible to merge TE sites and do genotyping in each sample? HOT 1
- Alu output didn't go through filter step? HOT 2
- xTea_long getting stuck, no errors, during wtpoa-cns, running HG38 HOT 2
- xTea output file understanding HOT 5
- About the case-control analysis in Illumina Short-read WGS Data HOT 3
- filtering HOT 4
- Will the coverage of BAM files affect the xTea results? HOT 3
- Input file for pseudogene insertion calling HOT 1
- visual inspection using IGV
- Merge clip and disc step error HOT 12
- Sporadic inconsistent errors with xtea_long HOT 5
- WES issues HOT 1
- interpretation of GENE_INFO in output file HOT 1
- Long read cannot find temp files HOT 1
- xTea Long_read SyntaxWarning HOT 6
- line 1133, IOError: [Errno 2] No such file or directory: 'null' HOT 1
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