Comments (1)
Hi,
No it's not a problem in the sense that the normalization procedure does not look at the time column it just assumes that the events in the FCS are ordered (i.e. the first event happened before the second event etc.).
It is up to you to concatenate the files in the right order though. If the time order is A.fcs, B.fcs, C.fcs, then you have to concatenate the files in that order as the normalization will not check the time and just assume the order is correct
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