Comments (4)
pfgherardini
commented on June 1, 2024
Hi Natalia,
Can you please share with me the data you are trying to debarcode so that I can reproduce and fix the issue on my system?
Thanks
Federico
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nsigal
commented on June 1, 2024
yes, definitely. Here are fcs file and the key code: https://drive.google.com/drive/folders/1xbDHLvl4nlJUjxj2lWr6aQ_9VTnGJrYK?usp=sharing
BTW I learned today that I if I change the setting from separation 0.3 to 0.1 and MD from 30 to something like 5, I am able to save the data.
Natalia
From: Pier Federico Gherardini <[email protected]>
Reply-To: ParkerICI/premessa <[email protected]>
Date: Monday, May 7, 2018 at 3:50 PM
To: ParkerICI/premessa <[email protected]>
Cc: Natalia Sigal <[email protected]>, Author <[email protected]>
Subject: Re: [ParkerICI/premessa] error while debarcoding (#13)
Hi Natalia,
Can you please share with me the data you are trying to debarcode so that I can reproduce and fix the issue on my system?
Thanks
Federico
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You are receiving this because you authored the thread.
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CNicholasMDA
commented on June 1, 2024
I too am having this issue, with parameters set at separation of 0.3 and MD at 30. I will try Natalia's modifications in a few minutes, but was wondering if there has been any new insight into this issue/error?
Thank you all!
-Courtney
UPDATE: separation of 0.1 and MD of 15 seemed to work in my case...
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pfgherardini
commented on June 1, 2024
I have fixed the problem in the latest release. The error was due to the fact that some samples contained only 1 or 2 cells, as the corresponding barcoding combinations were not used.
Thanks for reporting!
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