Comments (4)
Is this possible (yet)? I used pairtools merge
and would like to use distiller starting from pairs files. If not possible, I would love to hear suggestions for the best alternative!
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I don't think anything changed in this regard...
But actually this should work with the snakemake version of distiller! Probably you'd have to pretend that you have some fastq files... but that could be fixed in the future too, I think.
https://github.com/open2c/distiller-sm
Let me know if you'd like to try it, I could help you.
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Thanks @Phlya! I'll give it a shot.
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I think you might be the first person after me to try it after major updates I introduced recently, so might be a little rough around the edges. Hopefully nothing major, feel free to report any issues on github there.
The config should be exactly the same as for this distiller (plus an additional section if you want to automatically upload results to resgen), and I think file structure/naming at the later stages after pairs is also the same. Place your pairs files in the location they would have been generated, and describe the samples as you would if you had fastq files... and add some fake file paths to fastqs.
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Related Issues (20)
- where can I get the specific instructions of the software
- Error when trying to run on test dataset HOT 13
- process from 4DN?
- Running without duplicates filtering HOT 11
- Remove duplicate reads in sam/bam files
- Error: Process `map_parse_sort_chunks (library:HSPR2 run:lane1 chunk:0)` terminated with an error exit status (140) HOT 4
- distiller-nf run failed at executing process > 'bin_zoom_library_pairs‘ HOT 1
- Bug: split: output file suffixes exhausted
- Iterative mapping HOT 2
- Optional direct fastq downloads from ENA instead of sra-tools
- how memory limit gets specified in cluster using
- library (group) names can't be numerical
- bam files recycling
- update example cluster config - to match current distiller HOT 1
- Support of minimap2, bam mem2 and bwa aln HOT 1
- distiller 0.3.5 roadmap HOT 1
- filter with non-int columns
- which reference should I cite? HOT 1
- Help with "Error executing process > 'map_parse_sort_chunks...' HOT 4
- Skip deduplication for libraries with UMI
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