Comments (10)
Hello,
Are both Mash and mumer properly installed?
To check, please let me know the output from the following command:
$ dRep bonus test --check_dependencies
-Matt
from drep.
Hello,Matt
This is the output of the dependency check:
[zjs@www maxbin]$ /home/zjs/tools/drep/bin/dRep bonus test --check_dependencies
Loading work directory
Checking dependencies
mash.................................... all good (location = /home/zjs/hhf/soft/Mash/bin/mash)
nucmer.................................. all good (location = /home/zjs/hhf/soft/MUMmer3.23/nucmer)
checkm.................................. all good (location = /home/zjs/tools/pitchfork/deployment/bin/checkm)
ANIcalculator........................... all good (location = /home/zjs/hhf/soft/ANIcalculator_v1/ANIcalculator)
prodigal................................ all good (location = /usr/local/bin/prodigal)
centrifuge.............................. all good (location = /home/zjs/hhf/soft/centrifuge-1.0.3-beta/centrifuge)
And I still face the problem of "pandas.io.common.EmptyDataError: No columns to parse from file"
from drep.
Hmmm... that pandas error is what happens when it tries to read an empty dataframe. So this isn't really a problem with pandas, but a sign that something with dRep failed along the way.
Would you mind sending me the log file? It's located in the log
folder and is called logger.log
Thanks,
-Matt
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from drep.
Thank you for the log. This is strange- I'm not sure yet what the problem is.
Could you show me a list of what is in the folder:
...test_drep_out/data/MASH_files/
As well we as what is in the folder:
...test_drep_out/data/MASH_files/sketches/
?
Also, please let me know if any of the files in either of those folders is empty. I'm trying to figure out where Mash is messing up... thanks!
-Matt
from drep.
Thank you for your prompt reply
The /MASH_files/ was empty:
[zjs@www MASH_files]$ ll
total 0
-rwxrwxrwx. 1 root root 0 Oct 6 10:45 MASH_table.tsv
drwxrwxrwx. 1 root root 0 Oct 6 10:45 sketches_
and It is also empty in the folder of sketches
Thank you
And if you want to ask me for same other information ,you can contact me with email so i can reply immediately
I will copy this message and send to your gmail
from drep.
Hello,
OK, so it seems that mash isn't working properly. This could be an issue with dRep, or an issue with the program mash. Could you see if mash is working properly on it's own? For example, makes some sketches:
mash sketch maxbinout_W0P1.004.fasta MASH_files/maxbinout_W0P1.004.fasta.msh
Let me know if that command works and actually generates a file.
Thanks,
-Matt
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Hello,
I can run Mash on it's own like this:
mash sketch maxbinout_W0P1.004.fasta
And it will generate a file named:
maxbinout_W0P1.004.fasta.msh
But it failed when i type the order of :
mash sketch maxbinout_W0P1.004.fasta MASH_files/maxbinout_W0P1.004.fasta.msh
The Mash version was V1.1.1 .
from drep.
Hello,
Thats very strange that it's able to make the file normally, but not when it's to that specific folder... what is the error that it gives? Unfortunately, I've never encountered this issue before, but it sounds like an issue with permissions. May I ask what operating system you are using?
Using the "Skip_mash" parameter may be a way to get around this issue. Using that parameter makes the program take longer to run, but it will be just as accurate as if Mash was run, and in some cases even more accurate.
I'm worried that the underlying "permissions" issue may prevent other programs (like mummer, the program used is secondary clustering) from working as well. But if for some reason it's a mash-specific issue, using the "Skip_mash" parameter is a great idea.
Best,
-Matt
from drep.
Hallo,Matt
ohhh,now i can only run drep with skipmash.
I used CentOS system before and I am supposed to try it on Ubuntu
THanks for your greatest Help!
HU
from drep.
Related Issues (20)
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