Comments (1)
Hi Yijia,
Thanks for using my tool.
According to the error message, it seems that there are only 2 or less cells pass filter
. The non-zero median TSS is 1.43 and the median Fragment number is 8567.5, which is kinda low for quality control. If you look at the 10X mouse brain example dataset, you would see their median fragment number is 12,580 and enrichment at TSS is above 10. Therefore, I was wondering if it might be caused by the wrong genome annotation.
May I know whether your data is from human or mouse?
Sincerely,
Wenjing
from cellcano.
Related Issues (10)
- Data preprocessing HOT 4
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from cellcano.