Comments (3)
batch effect correction only applies to the low-dimensional embedding (adata.obsm["X_scANVI"]
) and whatever is derived from it (e.g. neighborhood graph, UMAP).
For all downstream analyses, we accounted for batch effects independently by including covariates in the linear models used for comparison.
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Thanks for your reply! I might have 2 follow-up questions about the expression counts in cellxgene data:
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there are three layers - X, which looks like normalized data, layer count and counts_length_scaled. Not sure if I understood this correctly, count is the raw count from original studies, counts_length_scaled is scaled count for only Smart-seq2 platform data (so raw counts was kept for other platforms?), and may I ask which normalization method is used for X?
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regarding batch effects, I think it can be added as cofactor in analysis like differential expression. I wonder for the dotplot of marker genes for cell-type annotation like in figure s1, did you also account batch effects in someway, or this is actually based on non-correction counts?
Thanks
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All you say is correct, X is simply scanpy.pp.normalize_total followed by scanpy.pp.log1p on the length-scaled counts
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the dotplots showning the cell-type markers were not adjusted for batch effects (we are also not trying to make any quantitative claims here)
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