Comments (16)
Can you try to run this without the .txt extension? I believe that the file name has to be gRANs-only2.fasta
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Hi Hyun-Hwan
I submitted an analysis last Friday, and the data was successfully uploaded. But I used the link to access the results a few times, and I got:
Processing
The analysis is waiting for its turn or is still going on. Please return to this page later.
Should I continue to wait for it or is there an issue that I should resubmit?
Thanks.
Benny
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@bennychang2 I deleted the link on Github.
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Thank you. I guess the link is accessible to everyone. Thank you very much.
Let me know whether the analysis is stalled or simply too many requests are being processed.
Thanks
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It's been three days; I wonder if I should submit the job again?
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@bennychang2, sorry for the delay, I was able to check the run, and it has been completed analysis. Are you still seeing the same message? If so, I can send the outputs to your email address. Let me know if you want.
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It showed that my webserver is ok; cloudfare is ok, but crispr.nrihub is down. So I could not access my results.
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The QC results showed low mappability values
24.84 and 27.06.
It does not seem to be good!
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I think I know what went wrong. I was trying to validate the CRISPRi library that I amplified after transforming clone DNA obtained from Addgene. The library was divided in 2 halves. 83987 has one half and 83988 has the other half.
In trying to validate I amplified the CRISPRi part of the DNA in the pool of clones and send for NGS. Because there are two pools (two halves), so each pool should have only half of the CRISPRi. But as an input I used the complete CRISPRi library. No wonder the mappabilities were low.
But now I do not know how I can get an idea of how the good the amplified half pool represent the original half library. Do you know what software can allow me to do that? Thanks.
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Related Issues (20)
- run_sgrna_quant fails HOT 3
- inconsistent between .fq.gz and .fastq? HOT 7
- calc_mappability HOT 1
- export join_count_and_design HOT 2
- plot_count_distribution add export option HOT 1
- gene_stat cpms HOT 2
- Clarity around logFC for gene_stats HOT 3
- Problem with gene-level statistic HOT 1
- Error in arising in run_sgrna_quant HOT 4
- How to handle paired-end fastqs HOT 5
- Cluster setup failed. 31 of 31 workers failed to connect. HOT 2
- run_sgrna_quant report the wrong sequences associated to sgRNA names HOT 2
- maximum guide length HOT 2
- Error in cb2_count() HOT 4
- Is it possible to analyze by inserting one mismatch guide RNA barcode? HOT 3
- Error in measure_gene_stats(sgrna_stat) HOT 3
- Interaction Terms and Complex Designs HOT 1
- CB2 vhat estimation with variable total read count
- Choice of count normalisation
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