Comments (6)
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Building off the RefSeq comment, you can make it clearer where you should start with the sequence. Ex. from the beginning of the primer, from the end of the primer, etc.
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Building off of Patrick's #3, I agree. Maybe you could have a small script here that autogenerates this csv for you since really the only information I needed to provide (at least for a single plate) was the (1) name, (2) number of variable positions, (3) forward sequence, (4) reverse sequence.
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Agreed @kadinaj. I'm going to make a branch and quickly address #1, #2, and #4 since those are very easy. #3 should be its own thing since it's a bit more involved.
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- Pass the file location of the GlobalParams.txt file when calling the program so that you can avoid needing to have it in the same folder as the script
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We should also make the Summary outputs include the actual codons at each position, since L and I (?) have two possible codons and that can very possibly make a difference in activity. (Or, if we don't use the 22c trick, it would be more critical.)
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Moved the 3rd issue here to a new stand-alone issue (#3).
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Closing for next round of updates.
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Related Issues (20)
- Update Matchmaking for Read Pairs HOT 1
- Run fails if "NNN" not detected within both the forward and reverse reads HOT 1
- Unhandled exception encountered: 'cannot convert float NaN to integer' HOT 2
- Implement different metric than Alignment Frequency. HOT 1
- Add functionality for visualizing sequence/function data. HOT 2
- Make setup.py file for easier install without needing to add to path. HOT 2
- Trim adaptor sequences if found in refseq HOT 2
- AaIndStart in RefSeq file cannot tolerate negative values HOT 2
- Make Alignment Penalties Tunable HOT 2
- Check GUI Still Works HOT 1
- Rows Sometimes Out of Order for Platemaps
- Reimplement Pytests HOT 1
- Lower int and float Sizes HOT 1
- Known Limitations
- Reads with Length Longer than Refseq Thrown Away
- Support Mixed Readlengths
- Refactor Alignment and Data Processing
- Random questions HOT 16
- Does evSeq support WSL? HOT 3
- Question/Error fixing HOT 7
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