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Comments (6)

kadinaj avatar kadinaj commented on August 15, 2024
  1. Building off the RefSeq comment, you can make it clearer where you should start with the sequence. Ex. from the beginning of the primer, from the end of the primer, etc.

  2. Building off of Patrick's #3, I agree. Maybe you could have a small script here that autogenerates this csv for you since really the only information I needed to provide (at least for a single plate) was the (1) name, (2) number of variable positions, (3) forward sequence, (4) reverse sequence.

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palmhjell avatar palmhjell commented on August 15, 2024

Agreed @kadinaj. I'm going to make a branch and quickly address #1, #2, and #4 since those are very easy. #3 should be its own thing since it's a bit more involved.

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kadinaj avatar kadinaj commented on August 15, 2024
  1. Pass the file location of the GlobalParams.txt file when calling the program so that you can avoid needing to have it in the same folder as the script

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palmhjell avatar palmhjell commented on August 15, 2024

We should also make the Summary outputs include the actual codons at each position, since L and I (?) have two possible codons and that can very possibly make a difference in activity. (Or, if we don't use the 22c trick, it would be more critical.)

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palmhjell avatar palmhjell commented on August 15, 2024

Moved the 3rd issue here to a new stand-alone issue (#3).

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palmhjell avatar palmhjell commented on August 15, 2024

Closing for next round of updates.

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