Comments (12)
Update: So the code from 2014 works loading lgen files but fails when trying trying to do a multi file merge using --merge-list
. The genotyping rate ends up being zero. Switching back to the 2016 version for the merge works.
from plink-ng.
Hi,
Can you send me a .lgen fileset to replicate this issue with? Thanks.
from plink-ng.
Do the gist files in the first post work?
from plink-ng.
The .map and .fam links work, but the .lgen link fails.
from plink-ng.
Right, sorry.
https://gist.github.com/74f13dc64fc3c3047c58
from plink-ng.
This should be fixed in the 13 March builds; let me know if you still have any problems.
from plink-ng.
Hi every one
I tried to make PED&MAP files by PLINK from three files that I made it .lgen ,.map &.fam So,error appeared
Error: Variant 'ARS-BFGL-BAC-27364' in .lgen file has 3+ different alleles.
So,the command that I used it ./plink --lfile rustaqi22 --cow --recode
Moreover,example below of rows of my .lgen file
Rustaqi 88 ARS-BFGL-BAC-10172 B B Rustaqi 88 ARS-BFGL-BAC-1020 A B Rustaqi 88 ARS-BFGL-BAC-10245 B B
Could any one advice how to deal with this point?
Thanks
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I'm using v1.9 from 20 Jun 2014. Would the fix of 13Mar2016 also account for .lgen import mistaking first two columns as marker id?
The log:
Working directory: /uufs/chpc.utah.edu/common/home/camp-group1/submissions/dbGAP/cll/denovo
Start time: Mon Mar 19 09:23:16 2018
Random number seed: 1521472996
64386 MB RAM detected; reserving 32193 MB for main workspace.
Processing .lgen file... Error: Variant '9999 101862' in .lgen file has 3+ different alleles.
The .lgen, wherein I've tried both tabs and spaces (shown):
9999 103355 200003 A G
9999 103389 200003 A A
9999 103590 200003 A A
9999 100563 200003 A G
9999 100698 200003 A A
9999 103720 200003 A G
9999 101182 200003 A A
9999 101414 200003 A G
9999 100682 200003 A A
9999 103789 200003 A A
from plink-ng.
Yes, this works in recent plink 1.9 builds.
from plink-ng.
Yes, thanks, I tried the "stable" release. The age old problem of misleading error messages. Turns out the data was malformed; "-" needs to be "0". I suspect this was not the case when last I used plink (2014).
from plink-ng.
$ plink --version
PLINK v1.90b6.9 64-bit (4 Mar 2019)
Error: 1 variant with 3+ alleles present.
- If you believe this is due to strand inconsistency, try --flip with
merge-merge.missnp.
(Warning: if this seems to work, strand errors involving SNPs with A/T or C/G
alleles probably remain in your data. If LD between nearby SNPs is high,
--flip-scan should detect them.) - If you are dealing with genuine multiallelic variants, we recommend exporting
that subset of the data to VCF (via e.g. '--recode vcf'), merging with
another tool/script, and then importing the result; PLINK is not yet suited
to handling them.
The version of PLINK software that I am using is 1.9, and I have encountered this issue as well. Could you please provide me with some advice?
from plink-ng.
Please post basic usage questions in the plink2-users Google group, not here.
from plink-ng.
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