particle size about imagedataextractor HOT 4 OPEN

Hi Alissa,

Sorry for the delayed response. The size of the example image in the imagedataextractor repository is 887x909. From the ImageJ example you posted, your image seems to be around half this size (492x480). This may explain the discrepancy you're seeing.

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Hi
Thank you so much for replying me.
I found that issue as well and the actual pixel is 127. However I tried to type nm/pixel for example 200/127. And I still got different results.

Last time you said that change these two line:
Conversion= nm / pixel
and
size = size *conversion

I wonder is that correct?
Thank you and take care!

Alissa

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Sorry for the confusion that the API is causing. When conversions are calculated automatically by imagedataextractor, they are returned in units of pixels / meters. This is why we divide by the conversion in the example notebook to obtain the results in meters or nanometers.

If you calculated the conversion manually in units of nanometers / pixel for example, then you would need to multiply by this value to obtain the result in nanometers.

Both ways are correct. I understand that this can cause confusion, so I may work on making this more clear in the API.

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Hi Batuhan,

I am confused by the same question as Alissa.

As you can see below, in the example image of size 887*909, 200nm can be converted to 127 pixels by Image J. Then I calculated the conversion manually in units of nanometers / pixels and multiplied by this value to get the result in nanometers, it output the average particle area is 1600.103 nm^2 which is much larger than the result of 647.872 shown in the example.

However, when I changed the conversion to pixels / nanometers, the output was pretty much as expected. I think it is a little bit tricky.

I am wondering if my code was wrong or I misunderstood your advice.

Looking forward to your reply. Have a lovely weekend.

Zeyu

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