Comments (7)
What is the format of your input data? Is it CellRanger? MTX or h5?
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MTX. It's not from cellranger but has the same format. I hadn't had issues with it before recent commits.
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Yes, so I have been updating the data parsers to retain gene_name and gene_id info, as well as feature_type info (if it's 10x Genomics v3 chemistry + ). But I still want it to work in the case that data is not available. Can you post the result of
$ head genes.tsv
on your input file?
from cellbender.
I've gone with cellranger 3 format, results of zcat features.tsv.gz | head
are:
ENSG00000251841.1 RNU6-1334P Gene Expression
ENSG00000184895.8 SRY Gene Expression
ENSG00000237659.1 RNASEH2CP1 Gene Expression
ENSG00000232195.1 TOMM22P2 Gene Expression
ENSG00000286130.1 AC006040.1 Gene Expression
ENSG00000129824.16 RPS4Y1 Gene Expression
CATG00000115038.1 CATG00000115038.1 Gene Expression
ENSG00000227289.2 HSFY3P Gene Expression
ENSG00000229163.2 NAP1L1P2 Gene Expression
CATG00000115039.1 CATG00000115039.1 Gene Expression
from cellbender.
Is it tab \t
delimited? Or are those spaces?
from cellbender.
They are tabs, just doesn't present well in this format...
from cellbender.
Hmm, okay, I see what happened. I think you were unlucky enough to pull the branch as I was in the middle of updating things. There was a typo of 'feature_type' instead of 'feature_types'. This should be fixed in the branch as it currently stands. Give that a try.
... the perils of working with a branch under development! :) But I am happy to be getting your feedback.
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Related Issues (20)
- cellbender v3.0 doesn't generate most of the output files, but doesn't have any errors
- "Trying to use CUDA, " \ AssertionError: Trying to use CUDA, but CUDA is not available.
- Number of cells after cellbender much more than number of cells from cellranger (filtered)
- New fileformat output from BD rhapsody HOT 1
- Should I keep decreasing the learning rate?
- Question about input h5 file HOT 1
- Computing the output in asynchronous chunks in parallel takes longer than 144 hours
- Unhandled division by zero
- Never mind
- Can't Computing target noise counts per gene for MCKP estimator HOT 1
- Importance of model loss
- Background Fraction HOT 2
- OOM posterior inference for chimeric sample even using --posterior-batch-size 1
- Not saving ckpt.tar.gz checkpoint HOT 7
- Fixed Single cell RNA seq HOT 1
- Cellbender on multiplexed chemistries
- RuntimeError: CUDA driver error: invalid argument - Google Container Registry (GCR)
- CellBender for .BAM file
- Increased number of UMIs per barcode after running remove-background HOT 1
- Mismatch between summary for algorithm convergence and learning curve
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