Comments (8)
from deblur.
Thank you for your prompt reply!
A positive filtering database is required according to the Deblur help documentation. How can I skip this?
from deblur.
from deblur.
Thank you so much!
By the way, may I ask you another question? Deblur performs quality control on each sample respectively. After this, is it that all the samples are mixed together to get the ASV table and representative sequence? Or do you get ASV separately for each sample and then merge these tables? Recently I have found that pooling samples or not will have a great impact on ASV numbers. Here is the link: benjjneb/dada2#1194. Do you have any thoughts on this?
Thanks again for your kind help.
from deblur.
from deblur.
Thank you for your answer! Compared to singleton, I think unique (i.e. species that occur in only one sample) is more likely to be a problem among different ASV algorithms. I found that some high numbers of ASVs only appear in one sample in DADA2, whilst all samples were parallel replicates. Deblur performed well in my test. So does Deblur do anything specific with this situation?
from deblur.
from deblur.
Thank you very much for your reply!
from deblur.
Related Issues (20)
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from deblur.