Comments (2)
I checked the m013330.abyss2.err. It said:
Running with max stack size of 65536 KB: abyss-bloom-dbg -k96 -q3 --kc=3 -v -b20G -H4 -j20 /public2/data/laixianxin/pacbio/ngs/cleandata/zhangqianqian_L1_353353.R1.clean.fastq.gz /public2/data/laixianxin/pacbio/ngs/cleandata/zhangqianqian_L1_353353.R2.clean.fastq.gz
abyss-bloom-dbg: /lib64/libstdc++.so.6: version GLIBCXX_3.4.21' not found (required by abyss-bloom-dbg) abyss-bloom-dbg: /lib64/libstdc++.so.6: version
GLIBCXX_3.4.20' not found (required by abyss-bloom-dbg)
abyss-bloom-dbg: /lib64/libstdc++.so.6: version `CXXABI_1.3.9' not found (required by abyss-bloom-dbg)
make[1]: *** [/public1/data/heke/pacbio/wengan-v0.2-bin-Linux/bin/abyss-pe:538: m013330.abyss2-1.fa] Error 1
make[1]: *** Deleting file 'm013330.abyss2-1.fa'
But i used command: strings /lib64/libstdc++.so.6|grep GLIBCXX
Then i found there indeed a GLIBCXX_3.4.21.
strings /lib64/libstdc++.so.6|grep GLIBCXX
GLIBCXX_3.4
GLIBCXX_3.4.1
GLIBCXX_3.4.2
GLIBCXX_3.4.3
GLIBCXX_3.4.4
GLIBCXX_3.4.5
GLIBCXX_3.4.6
GLIBCXX_3.4.7
GLIBCXX_3.4.8
GLIBCXX_3.4.9
GLIBCXX_3.4.10
GLIBCXX_3.4.11
GLIBCXX_3.4.12
GLIBCXX_3.4.13
GLIBCXX_3.4.14
GLIBCXX_3.4.15
GLIBCXX_3.4.16
GLIBCXX_3.4.17
GLIBCXX_3.4.18
GLIBCXX_3.4.19
GLIBCXX_3.4.20
GLIBCXX_3.4.21
GLIBCXX_3.4.22
GLIBCXX_3.4.23
GLIBCXX_3.4.24
GLIBCXX_3.4.25
GLIBCXX_3.4.26
GLIBCXX_3.4.27
GLIBCXX_3.4.28
GLIBCXX_3.4.29
GLIBCXX_DEBUG_MESSAGE_LENGTH
I don't know how i can fix this issue.
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I have successfully solved this problem. There is only libstdc++.so.6.0.19 on the compute node. I asked the root administrator to copy libstdc++.so.6.0.29 in my shared directory on the login node to /lib64/ on the compute node.
And I looked through the posts about assembly recommendations for low coverage data. My final parameters was "-M 1000-d 2-N 3-i 500,1000 ,2000,3000,4000,5000,6000,7000,8000,9000,10000,11000,12000,13000,14000,15000,20000" . Hope to get better result.
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