Relion 5 fails to reconstruct tomograms by asking for _rlnTomoMicrographNameEven label about relion HOT 10 CLOSED

I am facing this error too

from relion.

I am facing this error too

Hi Navya, I solved this problem. In the motion correction stage, at the 'Motion' panel, you need to input a 'gain.mrc' file as the blank for Relion to do the blanking.

After I input the gain.mrc file, the motion_corrected_tilt_series.star file has the '_rlnTomoMicrographNameEvn' and 'Odd' files showing up.
I hope this helps.

from relion.

Oh wow, where did you get the gain.mrc from?

from relion.

Oh wow, where did you get the gain.mrc from?

In the data I pulled out, there is a file called 'processing', in this file, I get the 'gain.mrc' file.

from relion.

Thanks! I tested with that but I still don't see the two columns in my corrected_tilt_series.star. Did you change anything else too?

from relion.

I only changed that bit. Is your file ending in '.mrc' or '.eer'?
Here are my parameters setting:
EER fractionation: 32 (this is according to the tutorial where if you are not using the .EER file, just leave it in 32)
write output in float16?: Yes
Save images for denoising?: Yes
save sum of power spectra?: Yes
sum power spectra every n frames: 4
Bfactor: 150
number of patches X,Y: 1, 1
group frames: 1
binning factor: 1
gain-reference image: gain.mrc (you need to browse to get the correct file in the correct path)
gain rotation: no
gatin flip: no
defect file: I leave this one empty
use RELION's own implementation?: yes

I hope this helps.

from relion.

Hi, it's .mrc and I supplied the gain reference image but I still end up with the same error. I am using AreTomo alignment, are you using that as well?

from relion.

Hi, it's .mrc and I supplied the gain reference image but I still end up with the same error. I am using AreTomo alignment, are you using that as well?

Hi, yes I use AreTomo. I suppose you may want to check if there is anything wrong in your previous steps...? I am doing the denoise, so I have also set the "save the images for denoise?" in the motion correction step as "Yes".

from relion.

I was able to fix it, Thank you!

from relion.

I was able to fix it, Thank you!

Happy to hear that! May I ask how did you fix that?

from relion.